English to French Science Patents Thrombospondin fragments as a diagnostic test for cancers

followed by purification of this TSP from the platelet-conditioned medium; if standard 96-well microtiter plates are

used, 75 ng of TSP-1 in 200 mL of phosphate-buffered saline can be added per well. Corresponding amounts (molar

or mass) of TSP fragments and/or peptides can be used instead, and are preferable, based on ease of preparation

and standardization. After binding the antigen to the immobilized surface, additional binding sites on the surface are

blocked by standard protocols (for example, incubation with bovine serum albumin then Tween, both in phosphate-

buffered saline).