diferulic acids (DiFA: 5,5'-DiFA, 8,5'-DiFA, 8-O-4'-DiFA, 8,5'-DiFAb)

Acide di-férulique

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Note added at 50 mins (2005-08-01 21:30:23 GMT)
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http://www.chemecol.org/meetings/Abstracts Ottawa 2004.pdf
(page 99 du document pdf)

De troutes façons, "ab"= antibody = anticorps. Anticorps anti-diF.

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Note added at 1 hr 7 mins (2005-08-01 21:48:05 GMT)
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http://www.inra.fr/bbt/2000/aout2000/Journal.htm Voir ici, le gène DIF joue un rôle dans le système immunitaire Ou là :
http://xray.bmc.uu.se/sbnet/nordlund.html

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Note added at 1 hr 11 mins (2005-08-01 21:51:10 GMT)
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C\'est un anticorps pégylé.

Arch Dermatol Res. 2004 Mar;295(10):434-41. Epub 2004 Jan 17. Related Articles, Links

A sensitive and restricted enzyme-linked immunosorbent assay for detecting a heterogeneous antibody population in serum from people suffering from a new variant of endemic pemphigus.

Abreu-Velez AM, Yepes MM, Patino PJ, Bollag WB, Montoya F Sr.

Institute for Molecular Medicine and Genetics, Medical College of Georgia, CB 2803, 1120 15th Street, GA 30912-2630, Augusta, USA, [email protected]

We recently described a new variant of endemic pemphigus foliaceus (EPF) in El Bagre, Colombia, that resembles Senear-Usher syndrome and identified autoantibodies to desmoglein 1 (Dsg1), as well as to multiple known and unknown antigens including plectins, in the serum of these patients. Here, we developed a cost-effective ELISA assay capable of detecting the heterogeneous antibody population observed in these EPF patients, and useful for serum epidemiological studies. A protein extract obtained from trypsin-digested fresh bovine skin and further purified on a concanavalin A matrix was used as antigen. This extract contains an important conformational epitope (a 45 kDa tryptic fragment of the Dsg1 ectodomain), which is recognized by antibodies in serum from patients with all varieties of pemphigus foliaceus (PF), and from half of those with pemphigus vulgaris with active clinical disease. The cut-off and threshold values were normalized using human serum obtained from both endemic and non-endemic areas for PF. The efficiency of this ELISA was tested using 600 serum samples from controls and patients diagnosed with EPF, non-endemic PF and other bullous diseases. The overall sensitivity and specificity of the assay were determined to be 95% and 72%, respectively, with reproducibilities of 98% (intraassay) and 95% (interassay). Comparing the ELISA with other tests to detect EPF autoantibodies, this ELISA was the most sensitive, followed by direct immunofluorescence (DIF), indirect immunofluorescence using anti-IgG4 monoclonal antibodies and immunoprecipitation (IP), respectively. The most specific assay was IP, followed by DIF. Immunoblotting to Dsg1 exhibited both poor sensitivity and poor specificity, although plectins were well visualized. We conclude that this ELISA is an excellent tool for field serological studies, allowing testing of multiple serum samples simultaneously and for detecting, with appropriate restriction and sensitivity, the heterogeneous antibody population seen in patients with this variant of EPF. Finally, autoantibody serum levels obtained with this ELISA correlated well with the clinical activity and extent of disease in patients with El Bagre EPF.
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Immunofluorescent testing is an important diagnostic adjunct in the assessment of immunologically mediated diseases of the skin and kidney. The 2 main methods of immunofluorescent testing are direct and indirect immunofluorescence. Direct immunofluorescence (DIF) involves the overlay of fluorescein-conjugated antibodies directed against immunoglobulins (IgG, IgM, and IgA) and complement fractions onto frozen sections of patient tissue.[1] Indirect immunofluorescence (IIF), by definition uses a linking antibody; in its common use, IIF refers to patient serum in concert with fluorescein-conjugated human anti-IgG applied to a mucosal substrate, such as guinea pig, monkey esophagus, or rat bladder.[2]
Direct and Indirect Immunofluorescence as a Diagnostic Adjunct in the Interpretation of Nonneoplastic Medical Lung Disease

Cynthia M. Magro, MD, Carl Morrison, MD, Amy Pope-Harman, MD, Susan K. Rothrauff, MPAS, PA-C, Patrick Ross, Jr, MD, PhD
Am J Clin Pathol 119(2):279-289, 2003. © 2003 American Society of Clinical Pathologists, Inc.
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Direct Fluorescent Antibody (DIF)
has high sensitivity;
is labor intensive;
is unsuitable for over 30 cases per day;
is the method of choice for confirmation of other assays;
accommodates all specimen types.
The Female Patient: Selected Guidelines

Abstracted by Elaine McClellan-Holm
South Med J 94(12):1146-1155, 2001. © 2001 Southern Medical Association

Screening for Cervical Cancer

American Geriatrics Society: Screening for cervical carcinoma in older women. J Am Geriatr Soc 2001; 49(5):655-657
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